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bioRxiv · 10.1101/2020.06.23.167163

The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish

Vong, Y. H.·Sivashanmugam, L.·Zaucker, A.·Jones, A.·Sampath, K.
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Abstract

The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs between species. In zebrafish, development of the germline is dependent on the specification, migration and proliferation of progenitors called the primordial germ cells (PGCs). PGC specification is dependent on a maternally provided cytoplasmic complex of ribonucleoproteins (RNPs), the germplasm. Here, we show that the conserved RNA-binding protein (RBP), Igf2bp3, has an essential role during germline development. Loss of Igf2bp3 leads to reduced germline RNA expression, and mis-regulated germline development. Maternal mutants affecting igf2bp3 exhibit abnormal PGC movement, maintenance and migration, and adult igf2bp3 mutants show a male biased sex ratio. Therefore, Igf2bp3 is required for germplasm components, and maintains correct development of the germline.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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Vong, Y. H., Sivashanmugam, L., Zaucker, A., Jones, A., Sampath, K.. 2020-06-23. The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish. https://doi.org/10.1101/2020.06.23.167163

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