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bioRxiv · 10.1101/2020.06.19.161679

Curvature effect of PE-included membrane on the behavior of cinnamycin on the membrane

Lee, S.-R.·Park, Y.·Park, J.-W.
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Abstract

The behavior of the cinnamycin on the biomimetic membrane was studied with respect to the curvature of the phosphatidylethanolamine(PE)-included membrane with the adhesion measured by the atomic force microscope(AFM). The membrane was formed through vesicle fusion on the hydrophobic surface of the sphere spheres, which was used to define the curvature of the membrane. The hydrophobicity was generated by the reaction of alkyl-silane and analyzed with the X-ray photoelectron spectrometer. The cinnamycin, immobilized covalently to the AFM tip coated with 1-mercapto-1-undecanol that was observed inert to any adhesion to the membrane, showed that the adhesion became stronger with the increase in the curvature. The correlation between the adhesion and the curvature was linearly proportional. Previously, it was found that the cinnamycin was bound to PE headgroup and the binding was enhanced by the interaction of the hydrophobic area located at one side of the cinnamycin. Therefore, the linear proportionality seems to suggest that the interaction is related to the one dimensional orientation of the binding. Statement of SignificanceThe behavior of the cinnamycin was studied on the phosphatidylethanolamine(PE)-included membrane with respect to the curvature of the membrane. The cinnamycin, immobilized covalently to the atomic force microscope, showed that the adhesion became stronger linearly with the increase in the curvature. Previously, it was found that the specific binding between the cinnamycin and PE headgroup was enhanced by the interaction of the hydrophobic area located at one side of the cinnamycin. Therefore, the linear proportionality seems to suggest that the interaction is related to the one dimensional orientation of the binding.

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Lee, S.-R., Park, Y., Park, J.-W.. 2020-06-20. Curvature effect of PE-included membrane on the behavior of cinnamycin on the membrane. https://doi.org/10.1101/2020.06.19.161679

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