Single-molecule live cell imaging of the Smc5/6 DNA repair complex

Etheridge, T. J.; Villahermosa, D.; Irmisch, A.; Watson, A. T.; Herbert, A.; Dang, H. Q.; Osborne, M. A.; Oliver, A. W.; Carr, A. M.; Murray, J. M.

doi:10.1101/2020.06.19.148106

bioRxiv · 10.1101/2020.06.19.148106

Single-molecule live cell imaging of the Smc5/6 DNA repair complex

Etheridge, T. J.·Villahermosa, D.·Irmisch, A.·Watson, A. T.·Herbert, A.·Dang, H. Q.·Osborne, M. A.·Oliver, A. W.·Carr, A. M.·Murray, J. M.

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Abstract

The essential Smc5/6 complex is required in response to replication stress and is best known for ensuring the fidelity of homologous recombination. Using single-molecule tracking in live fission yeast to investigate Smc5/6 chromatin association, we show that Smc5/6 is chromatin associated in unchallenged cells and this depends on the non-SMC protein Nse6. We define a minimum of two Nse6-dependent sub-pathways, one of which requires the BRCT-domain protein Brc1. Using defined mutants in genes encoding the core Smc5/6 complex subunits we show that the Nse3 double-stranded DNA binding activity and the arginine fingers of the two Smc5/6 ATPase binding sites are critical for chromatin association. Interestingly, disrupting the ssDNA binding activity at the hinge region does not prevent chromatin association but leads to elevated levels of gross chromosomal rearrangements during replication restart. This is consistent with a downstream function for ssDNA binding in regulating homologous recombination.

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Etheridge, T. J., Villahermosa, D., Irmisch, A., Watson, A. T., Herbert, A., Dang, H. Q., Osborne, M. A., Oliver, A. W., Carr, A. M., Murray, J. M.. 2020-06-20. Single-molecule live cell imaging of the Smc5/6 DNA repair complex. https://doi.org/10.1101/2020.06.19.148106

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