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bioRxiv · 10.1101/2020.06.17.158311

Loss of O-GlcNAcylation on MeCP2 Thr 203 Leads to Neurodevelopmental Disorders

Cheng, J.·Zhao, Z.·Chen, L.·Du, R.·Wu, Y.·Zhu, Q.·Fan, M.·Duan, X.·Wu, H.
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Abstract

Mutations of the X-linked methyl-CpG-binding protein 2 (MECP2) gene in humans are responsible for most cases of Rett syndrome (RTT), an X-linked progressive neurological disorder. While genome-wide screens in clinical trials reveal several putative RTT-associated mutations on MECP2, their causative relevance regarding the functional regulation of MeCP2 on the etiologic sites at the protein level require more evidence. In this study, we demonstrate that MeCP2 is dynamically modified by O-linked-{beta}-N-acetylglucosamine (O-GlcNAc) at threonine 203 (T203), an etiologic site in RTT patients. Disruption of the O-GlcNAcylation of MeCP2 specifically at T203 impairs dendrite development and spine maturation in cultured hippocampal neurons, and disrupts neuronal migration, dendritic spine morphogenesis and dysfunction of synaptic transmission in the developing and juvenile mouse cerebral cortex. Mechanistically, genetic disruption of O-GlcNAcylation at T203 on MeCP2 decreases neuronal activity-induced induction of Bdnf transcription. Our study highlights the critical role of MeCP2 T203 O-GlcNAcylation in neural development and synaptic transmission potentially via BDNF.

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Cheng, J., Zhao, Z., Chen, L., Du, R., Wu, Y., Zhu, Q., Fan, M., Duan, X., Wu, H.. 2020-06-18. Loss of O-GlcNAcylation on MeCP2 Thr 203 Leads to Neurodevelopmental Disorders. https://doi.org/10.1101/2020.06.17.158311

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