Genome sequence of Hydrangea macrophylla and its application in analysis of the double flower phenotype

Nashima, K.; Shirasawa, K.; Ghelfi, A.; Hirakawa, H.; Isobe, S.; Suyama, T.; Wada, T.; Kurokura, T.; Uemachi, T.; Azuma, M.; Akutsu, M.; Kodama, M.; Nakazawa, Y.; Namai, K.

doi:10.1101/2020.06.14.151431

bioRxiv · 10.1101/2020.06.14.151431

Genome sequence of Hydrangea macrophylla and its application in analysis of the double flower phenotype

Nashima, K.·Shirasawa, K.·Ghelfi, A.·Hirakawa, H.·Isobe, S.·Suyama, T.·Wada, T.·Kurokura, T.·Uemachi, T.·Azuma, M.·Akutsu, M.·Kodama, M.·Nakazawa, Y.·Namai, K.

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Abstract

Owing to its high ornamental value, the double flower phenotype of hydrangea (Hydrangea macrophylla) is one of its most important traits. In this study, genome sequence information was obtained to explore effective DNA markers and the causative genes for double flower production in hydrangea. Single molecule real-time sequencing data followed by a HiC analysis was employed. The resultant haplotype-phased sequences consisted of 3,779 sequences (2.256 Gb in length and N50 of 1.5 Mb), and 18 pseudomolecules comprising 1.08 Gb scaffold sequences along with a high-density SNP genetic linkage map. Using the genome sequence data obtained from two breeding populations, the SNPs linked to double flower loci (Djo and Dsu), were discovered for each breeding population. DNA markers J01 linked to Djo and S01 linked to Dsu were developed, and these could be used successfully to distinguish the recessive double flower allele for each locus respectively. The LEAFY gene was suggested as the causative gene for Dsu, since frameshift was specifically observed in double flower accession with dsu. The genome information obtained in this study will facilitate a wide range of genomic studies on hydrangea in the future.

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Nashima, K., Shirasawa, K., Ghelfi, A., Hirakawa, H., Isobe, S., Suyama, T., Wada, T., Kurokura, T., Uemachi, T., Azuma, M., Akutsu, M., Kodama, M., Nakazawa, Y., Namai, K.. 2020-06-15. Genome sequence of Hydrangea macrophylla and its application in analysis of the double flower phenotype. https://doi.org/10.1101/2020.06.14.151431

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