Monitoring S. pombe genome stress by visualizing end-binding protein Ku

Jones, C. E.; Forsburg, S. L.

doi:10.1101/2020.06.12.149054

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bioRxiv · 10.1101/2020.06.12.149054

Monitoring S. pombe genome stress by visualizing end-binding protein Ku

Jones, C. E.·Forsburg, S. L.

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Abstract

Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.

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Jones, C. E., Forsburg, S. L.. 2020-06-12. Monitoring S. pombe genome stress by visualizing end-binding protein Ku. https://doi.org/10.1101/2020.06.12.149054

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