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bioRxiv · 10.1101/2020.06.12.147884

REALM: AO-based localization microscopy deep in complex tissue

Siemons, M. E.·Hanemaaijer, N. A. K.·Kole, M. H. P.·Kapitein, L. C.
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Abstract

Performing Single-Molecule Localization Microscopy (SMLM) in complex biological tissues, where sample-induced aberrations hamper detection and localization, has remained a challenge. Here we establish REALM (Robust and Effective Adaptive Optics in Localization Microscopy), which corrects aberrations of [≤]1 rad RMS using 297 frames of blinking molecules to improve single-molecule localization. We demonstrate this method by resolving the periodic cytoskeleton of the axon initial segment at 50 m depth in brain tissue.

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Siemons, M. E., Hanemaaijer, N. A. K., Kole, M. H. P., Kapitein, L. C.. 2020-06-12. REALM: AO-based localization microscopy deep in complex tissue. https://doi.org/10.1101/2020.06.12.147884

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