CyTOF analysis identifies unusual immune cells in urine of BCG-treated bladder cancer patients

Castellano, E.; Samba, C.; Esteso, G.; Simpson, L. J.; Vendrame, E.; Garcia-Cuesta, E. M.; Lopez-Cobo, S.; Alvarez-Maestro, M.; Linares, A.; Leibar, A.; Ranganath, T.; Reyburn, H. T.; Martinez-Pineiro, L.; Blish, C.; Vales-Gomez, M.

doi:10.1101/2020.06.12.147710

bioRxiv · 10.1101/2020.06.12.147710

CyTOF analysis identifies unusual immune cells in urine of BCG-treated bladder cancer patients

Castellano, E.·Samba, C.·Esteso, G.·Simpson, L. J.·Vendrame, E.·Garcia-Cuesta, E. M.·Lopez-Cobo, S.·Alvarez-Maestro, M.·Linares, A.·Leibar, A.·Ranganath, T.·Reyburn, H. T.·Martinez-Pineiro, L.·Blish, C.·Vales-Gomez, M.

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Abstract

High grade non-muscle-invasive bladder tumours are treated with transurethral resection followed by recurrent intravesical instillations of Bacillus Calmette Guerin (BCG). Although bladder cancer patients respond well to BCG, important questions remain unanswered, including how to identify at early stages non-responder patients and patients at risk to abandon the treatment. Here, we analysed the cells released into the urine of bladder cancer patients longitudinally 3-7 days after BCG instillations. Mass cytometry (CyTOF) analyses revealed that most cells were granulocytes and monocytes rather than effector lymphocytes, and most expressed activation markers. A novel population of CD15+CD66b+CD14+ CD16+ cells was very abundant in several samples and expression of these markers was confirmed using flow cytometry and qPCR. Samples of patients with a stronger inflammatory response contained more cells in urine; however, this was not due to haematuria, as the proportions of the cell populations observed were different from blood. We provide the proof-of-concept for a new approach to analyse samples that may help classify patients and identify those at risk of BCG infection and other unwanted BCG-related events.

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Castellano, E., Samba, C., Esteso, G., Simpson, L. J., Vendrame, E., Garcia-Cuesta, E. M., Lopez-Cobo, S., Alvarez-Maestro, M., Linares, A., Leibar, A., Ranganath, T., Reyburn, H. T., Martinez-Pineiro, L., Blish, C., Vales-Gomez, M.. 2020-06-16. CyTOF analysis identifies unusual immune cells in urine of BCG-treated bladder cancer patients. https://doi.org/10.1101/2020.06.12.147710

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