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bioRxiv · 10.1101/2020.06.11.121418

Lyophilized cell-free systems display tolerance to organic solvent exposure

Lee, M. S.·Raig, R. M.·Gupta, M. K.·Lux, M. W.
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Abstract

Cell-free systems offer a powerful way to deliver biochemical activity to the field without cold chain storage. These systems are capable of sensing as well as biosynthesis of useful molecules at the point of need. So far, cell-free protein synthesis (CFPS) reactions have been studied as aqueous solutions in test tubes or absorbed into paper or cloth. Embedding biological functionality into broadly-used materials, such as plastic polymers, represents an attractive goal. Unfortunately, this goal has for the most part remained out of reach, presumably due to the fragility of biological systems outside of aqueous environments. Here, we describe a surprising and useful feature of lyophilized cell-free lysate systems: tolerance to a variety of organic solvents. Screens of individual CFPS reagents and different CFPS methods reveal that solvent tolerance varies by CFPS reagent composition. Tolerance to suspension in organic solvents may facilitate the use of polymers to deliver dry cell-free reactions in the form of coatings or fibers, or allow dosing of analytes or substrates dissolved in non-aqueous solvents, among other processing possibilities.Competing Interest StatementThe authors have declared no competing interest.AbbreviationsCFPScell-free protein synthesisGFPgreen fluorescent proteinDCMdichloromethaneDMFdimethylformamideDMSOdimethylsulfoxideTHFtetrahydrofuranView Full Text

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Lee, M. S., Raig, R. M., Gupta, M. K., Lux, M. W.. 2020-06-12. Lyophilized cell-free systems display tolerance to organic solvent exposure. https://doi.org/10.1101/2020.06.11.121418

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