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bioRxiv · 10.1101/2020.06.06.138305

Bioengineering a light-responsive encapsulin nanoreactor: a potential tool for photodynamic therapy

Diaz, D.·Vidal, X.·Sunna, A.·Care, A.
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Abstract

Encapsulins, a prokaryotic class of self-assembling protein nanocompartments, are being re-engineered to serve as ‘nanoreactors’ for the augmentation or creation of key biochemical reactions. However, approaches that allow encapsulin nanoreactors to be functionally activated with spatial and temporal precision is lacking. We report the construction of a light-responsive encapsulin nanoreactor for “on-demand” production of reactive oxygen species (ROS). Herein, encapsulins were loaded with the fluorescent flavoprotein mini-Singlet Oxygen Generator (miniSOG), a biological photosensitizer that is activated by blue-light to generate ROS, primarily singlet oxygen (1O2). We established that the nanocompartments stably encased miniSOG, and in response to blue-light were able to mediate the photoconversion of molecular oxygen into ROS. Using an in vitro model of lung cancer, ROS generated by the nanoreactor was shown to trigger photosensitized oxidation reactions that exerted a toxic effect on tumour cells, suggesting utility in photodynamic therapy. This encapsulin nanoreactor thus represents a platform for the light-controlled initiation and/or modulation of ROS-driven processes in biomedicine and biotechnology.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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Diaz, D., Vidal, X., Sunna, A., Care, A.. 2020-06-07. Bioengineering a light-responsive encapsulin nanoreactor: a potential tool for photodynamic therapy. https://doi.org/10.1101/2020.06.06.138305

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