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bioRxiv · 10.1101/2020.06.02.130716

STAT3 Serine phosphorylation is required for TLR4 metabolic reprogramming and IL-1β expression

Abstract

Detection of microbial components such as lipopolysaccharide (LPS) by Toll-like receptor (TLR)-4 expressed on macrophages induces a robust pro-inflammatory response which has recently been shown to be dependent on metabolic reprogramming 1, 2, 3, 4. These innate metabolic changes have been compared to the Warburg effect (also known as aerobic glycolysis) described in tumour cells 5, 6. However, the mechanisms by which TLR4 activation leads to mitochondrial and glycolytic reprogramming remain unknown. Here we show that TLR4 activation induces a signalling cascade recruiting TRAF6 and TBK-1, while TBK-1 phosphorylates STAT3 on S727. Using a genetically engineered mouse model incapable of undergoing STAT3 Ser727 phosphorylation, we show both ex vivo and in vivo that STAT3 Ser727 phosphorylation is critical for LPS-induced glycolytic reprogramming, the production of the central immune-metabolite succinate and inflammatory cytokine production in a model of LPS-induced inflammation. Our study identifies non-canonical STAT3 activation as the crucial signalling intermediary for TLR4-induced glycolysis, macrophage metabolic reprogramming and inflammation.

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BibTeXRIS

Balic, J., Albargy, H., Luu, K., Kirby, F., Jayasekara, W., Mansell, F., Garama, D., Baschuk, N., De Nardo, D., Louis, C., Humphries, F., Fitzgerald, K. A., Latz, E., Gough, D., Mansell, A.. 2020-06-02. STAT3 Serine phosphorylation is required for TLR4 metabolic reprogramming and IL-1β expression. https://doi.org/10.1101/2020.06.02.130716

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