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bioRxiv · 10.1101/2020.06.02.128900

A physiological role for GABAA receptor desensitization - induction of long-term potentiation at inhibitory synapses

Field, M.·Thomas, P.·Smart, T.
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Abstract

GABAA receptors (GABAARs) are pentameric ligand-gated ion channels distributed throughout the brain where they mediate synaptic and tonic inhibition. Following activation, these receptors undergo desensitization which involves entry into long-lived agonist-bound closed states. Although the kinetic effects of this state are recognised and its structural basis has been uncovered, the physiological impact of desensitization on inhibitory neurotransmission remains unknown. Here we describe an enduring new form of long-term potentiation at inhibitory synapses that elevates synaptic current amplitude for 24 hrs following desensitization of GABAARs in response to prolonged agonist exposure or allosteric modulation. Using receptor mutants and allosteric modulators we demonstrate that desensitization of GABAARs facilitates their phosphorylation by PKC, which increases the number of receptors at inhibitory synapses. These observations provide a new physiological relevance to the desensitized state of GABAARs, acting as a signal to regulate the efficacy of inhibitory synapses during prolonged periods of inhibitory neurotransmission.

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Field, M., Thomas, P., Smart, T.. 2020-06-03. A physiological role for GABAA receptor desensitization - induction of long-term potentiation at inhibitory synapses. https://doi.org/10.1101/2020.06.02.128900

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