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bioRxiv · 10.1101/2020.06.01.128322

Muscle fiber type differences in nitrate and nitrite storage and nitric oxide signaling in rats

Long, G. M.·Gray, D. A.·Troutman, A. D.·Fisher, A. J.·Brown, M. B.·Coggan, A. R.
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Abstract

Recent studies have emphasized the importance of the nitric oxide synthase (NOS)-independent, nitrate (NO3-) [->] nitrite (NO2-) [->] nitric oxide (NO) pathway in skeletal muscle. In particular, it has been hypothesized that this pathway is especially active in type II, or fast-twitch, muscle fibers, necessitating greater NO3- and NO2- storage. We therefore measured NO3- and NO2- concentrations in the predominantly fast-twitch vastus lateralis and predominantly slow-twitch soleus muscles of rats. Contrary to the above hypothesis, we found that NO3- and NO2- concentrations were 3.4-fold and 1.8-fold higher, respectively, in the soleus. On the other hand, NO signaling (i.e., cyclic guanosine monophosphate (cGMP) level) was comparable in the two muscles. Although the physiological significance of these observations remains to be determined, we speculate that NO production via the NO3- [->] NO2- [->] NO pathway is normally higher in slow-twitch muscles, thus helping compensate for their inherently lower NOS activity.

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Long, G. M., Gray, D. A., Troutman, A. D., Fisher, A. J., Brown, M. B., Coggan, A. R.. 2020-06-02. Muscle fiber type differences in nitrate and nitrite storage and nitric oxide signaling in rats. https://doi.org/10.1101/2020.06.01.128322

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