SARS-CoV-2 infects and induces cytotoxic effects in human cardiomyocytes

Bojkova, D.; Wagner, J.; Shumliakivska, M.; Aslan, G.; Saleem, U.; Hansen, A.; Luxan, G.; Guenther, S.; Pham, M. D.; Krishnan, J.; Harter, P.; Ermel, U.; Frangakis, A. S.; Zeiher, A.; Milting, H.; Cinatl, J.; Dendorfer, A.; Eschenhagen, T.; Ciesek, S.; Dimmeler, S.

doi:10.1101/2020.06.01.127605

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bioRxiv · 10.1101/2020.06.01.127605

SARS-CoV-2 infects and induces cytotoxic effects in human cardiomyocytes

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Abstract

BackgroundThe coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has emerged as global pandemic. SARS-CoV-2 infection can lead to elevated markers of cardiac injury associated with higher risk of mortality in COVID-19 patients. It is unclear whether cardiac injury may have been caused by direct infection of cardiomyocytes or is mainly secondary to lung injury and inflammation. Here we investigate whether human cardiomyocytes are permissive for SARS-CoV-2 infection. MethodsInfection was induced by two strains of SARS-CoV-2 (FFM1 and FFM2) in human induced pluripotent stem cells-derived cardiomyocytes (hiPS-CM) and in two models of human cardiac tissue. ResultsWe show that SARS-CoV-2 infects hiPS-CM as demonstrated by detection of intracellular double strand viral RNA and viral spike glycoprotein protein expression. Increasing concentrations of virus RNA are detected in supernatants of infected cardiomyocytes, which induced infections in CaCo-2 cell lines documenting productive infections. SARS-COV-2 infection induced cytotoxic and pro-apoptotic effects and abolished cardiomyocyte beating. RNA sequencing confirmed a transcriptional response to viral infection as demonstrated by the up-regulation of genes associated with pathways related to viral response and interferon signaling, apoptosis and reactive oxygen stress. SARS-CoV-2 infection and cardiotoxicity was confirmed in a iPS-derived human 3D cardiosphere tissue models. Importantly, viral spike protein and viral particles were detected in living human heart slices after infection with SARS-CoV-2. ConclusionsThe demonstration that cardiomyocytes are permissive for SARS-CoV-2 infection in vitro warrants the further in depth monitoring of cardiotoxic effects in COVID-19 patients. Clinical PerspectiveO_ST_ABSWhat is New?C_ST_ABSO_LIThis study demonstrates that human cardiac myocytes are permissive for SARS-CoV-2 infection. C_LIO_LIThe study documents that SARS-CoV-2 undergoes a full replicatory circle and induces a cytotoxic response in cardiomyocytes. C_LIO_LIInfection was confirmed in two cardiac tissue models, including living human heart slices. C_LI What are the Clinical Implications?O_LIThe study may provide a rational to explain part of the cardiotoxicity observed in COVID-19 patients C_LIO_LIThe demonstration of direct cardiotoxicity induced by SARS-CoV-2 warrants an in depth further analysis of cardiac tissue of COVID-19 patients and a close monitoring for putative direct cardiomyocyte injury. C_LIO_LIThe established models can be used to test novel therapeutic approaches targeting COVID-19. C_LI

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Bojkova, D., Wagner, J., Shumliakivska, M., Aslan, G., Saleem, U., Hansen, A., Luxan, G., Guenther, S., Pham, M. D., Krishnan, J., Harter, P., Ermel, U., Frangakis, A. S., Zeiher, A., Milting, H., Cinatl, J., Dendorfer, A., Eschenhagen, T., Ciesek, S., Dimmeler, S.. 2020-06-01. SARS-CoV-2 infects and induces cytotoxic effects in human cardiomyocytes. https://doi.org/10.1101/2020.06.01.127605

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