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bioRxiv · 10.1101/2020.05.26.116335

N-Aryl Pyrido Cyanine derivatives: nuclear and organelle DNA markers for two-photon and super-resolution imaging

Uno, K.·Sugimoto, N.·Sato, Y.
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Abstract

Live cell imaging using DNA-binding fluorescent probes is an essential molecular tool in various biological and biomedical fields. The major challenges in currently used DNA probes are to avoid UV light photo-excitation with high DNA selectivity and cell-permeability and are the availability of the cutting-edge imaging techniques such as a super-resolution microscopy. Herein we report new orange to red fluorogenic DNA probes having N-aryl pyrido cyanine (PC) moiety as a basic skeleton. Their DNA selectivity and cell-permeabilities are so high that organelle DNA as well as nuclear DNA can be clearly stained in various cell types and plant tissues with wash-free manner. PC dyes are also compatible with a stimulated emission depletion fluorescent lifetime imaging microscopy (STED-FLIM) for super-resolution imaging as well as two-photon microscopy for deep tissue imaging, should release the utilization limitation of synthetic DNA probes.

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Uno, K., Sugimoto, N., Sato, Y.. 2020-05-27. N-Aryl Pyrido Cyanine derivatives: nuclear and organelle DNA markers for two-photon and super-resolution imaging. https://doi.org/10.1101/2020.05.26.116335

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