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bioRxiv · 10.1101/2020.05.25.114256

Reliable identification of protein-protein interactions by crosslinking mass spectrometry

Lenz, S.·Sinn, L. R.·O'Reilly, F. J.·Fischer, L.·Wegner, F.·Rappsilber, J.
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Abstract

Crosslinking mass spectrometry is widening its scope from structural analyzes of purified multi-protein complexes towards systems-wide analyzes of protein-protein interactions. Assessing the error in these large datasets is currently a challenge. Using a controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate a reliable false-discovery rate estimation procedure for protein-protein interactions identified by crosslinking mass spectrometry.

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Lenz, S., Sinn, L. R., O'Reilly, F. J., Fischer, L., Wegner, F., Rappsilber, J.. 2020-05-25. Reliable identification of protein-protein interactions by crosslinking mass spectrometry. https://doi.org/10.1101/2020.05.25.114256

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