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bioRxiv · 10.1101/2020.05.21.108175

The NHEJ Repair of DNA Double Strand Breaks in Physcomitrella patens Depends on the Kleisin NSE4 of the SMC5/6 Complex.

Vagnerova, R.·Hola, M.·Angelis, K. J.
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Abstract

Structural maintenance of chromosomes (SMC) complexes are involve in cohesion, condensation and maintenance of genome stability. Based on the sensitivity of mutants to genotoxic stress the SMC5/6 complex is thought to play imminent role in DNA stabilization during repair by encircling DNA at the site of lesion by bridging the heteroduplex of SMC5 and SMC6 by non SMC kleisin components NSE1, 3 and 4. In this study, we tested how formation of the SMC5/6 circular structure affects mutant sensitivity to genotoxic stress, kinetics of DSB repair and insertion mutagenesis. In the moss Physcomitrella patens SMC6 and NSE4 are essential single copy genes and this is why we used blocking of transcription to reveal their mutated phenotype. Even slight attenuation of transcription by dCas9 binding was enough to obtain stable lines with DSB repair defect and specific bleomycin sensitivity. Whereas survival after bleomycin or MMS treatment fully depends on active SMC6, NSE4 has little or negligible effect. We conclude that whereas circularization of SMC5/6 provided by the kleisin NSE4 is indispensable for the immediate NHEJ DSB repair response, other functions associated with SMC5/6 complex are critical to survive DNA damage.

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Vagnerova, R., Hola, M., Angelis, K. J.. 2020-05-22. The NHEJ Repair of DNA Double Strand Breaks in Physcomitrella patens Depends on the Kleisin NSE4 of the SMC5/6 Complex.. https://doi.org/10.1101/2020.05.21.108175

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