Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in Saccharomyces cerevisiae as a vaccine candidate

Arora, K.; Rastogi, R.; Arora, N. M.; Parashar, D.; Paliwal, J.; Naqvi, A.; Srivastava, A.; Singh, S. K.; Kalyanaraman, S.; Potdar, S.; Kumar, D.; Arya, V. B.; Bansal, S.; Rautray, S.; Singh, I.; Fengade, P. S.; Kumar, B.; Kundu, P. K.

doi:10.1101/2020.05.18.099234

bioRxiv · 10.1101/2020.05.18.099234

Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in Saccharomyces cerevisiae as a vaccine candidate

Arora, K.·Rastogi, R.·Arora, N. M.·Parashar, D.·Paliwal, J.·Naqvi, A.·Srivastava, A.·Singh, S. K.·Kalyanaraman, S.·Potdar, S.·Kumar, D.·Arya, V. B.·Bansal, S.·Rautray, S.·Singh, I.·Fengade, P. S.·Kumar, B.·Kundu, P. K.

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Abstract

Spike, Envelope and Membrane proteins from the SARS CoV-2 virus surface coat are important vaccine targets. We hereby report recombinant co-expression of the three proteins (Spike, Envelope and Membrane) in a engineered Saccharomyces cerevisiae platform (D-Crypt) and their self-assembly as Virus-like particle (VLP). This design as a multi-antigenic VLP for SARS CoV-2 has the potential to be a scalable vaccine candidate. The VLP is confirmed by transmission electron microscopy (TEM) images of the SARS CoV-2, along with supportive HPLC, Dynamic Light Scattering (DLS) and allied analytical data. The images clearly outline the presence of a "Corona" like morphology, and uniform size distribution.

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Arora, K., Rastogi, R., Arora, N. M., Parashar, D., Paliwal, J., Naqvi, A., Srivastava, A., Singh, S. K., Kalyanaraman, S., Potdar, S., Kumar, D., Arya, V. B., Bansal, S., Rautray, S., Singh, I., Fengade, P. S., Kumar, B., Kundu, P. K.. 2020-05-19. Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in Saccharomyces cerevisiae as a vaccine candidate. https://doi.org/10.1101/2020.05.18.099234

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