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bioRxiv · 10.1101/2020.05.17.100735

Genome-wide effects of the antimicrobial peptide apidaecin on translation termination

Vazquez-Laslop, N.·Mankin, A.·FLorin, T.·Mangano, K.·Shao, X.·Klepacki, D.·Chelysheva, I.·Ignatova, Z.·Gao, Y.
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Abstract

Biochemical studies suggested that the antimicrobial peptide apidaecin (Api) inhibits protein synthesis by binding in the nascent peptide exit tunnel and trapping the release factor associated with a terminating ribosome. The mode of Api action in bacterial cells had remained unknown. Here, genome-wide analysis revealed that Api arrests translating ribosomes at stop codons and causes pronounced queuing of the trailing ribosomes. By sequestering the available release factors, Api promotes pervasive stop codon bypass, leading to expression of proteins with C-terminal extensions. Api-mediated translation arrest leads to futile activation of the ribosome rescue systems. Understanding the unique mechanism of Api action in living cells may facilitate development of new medicines and research tools for genome exploration.

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Vazquez-Laslop, N., Mankin, A., FLorin, T., Mangano, K., Shao, X., Klepacki, D., Chelysheva, I., Ignatova, Z., Gao, Y.. 2020-05-20. Genome-wide effects of the antimicrobial peptide apidaecin on translation termination. https://doi.org/10.1101/2020.05.17.100735

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