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bioRxiv · 10.1101/2020.05.14.095158

Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen-enrichment sequencing (PenSeq)

Lin, X.·Song, T.·Fairhead, S.·Witek, K.·Jouet, A.·Jupe, F.·Witek, A. I.·Vleeshouwers, V. G. A. A.·Hein, I.·Jones, J. D. G.
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Abstract

O_LIPotato late blight, caused by the oomycete pathogen Phytophthora infestans, significantly hampers potato production. Recently, a new Resistance to Phytophthora infestans (Rpi) gene, Rpi-amr1, was cloned from a wild Solanum species, Solanum americanum. Identification of the corresponding recognized effector (Avirulence, or Avr) genes from P. infestans is key to elucidating their naturally occurring sequence variation, which in turn informs the potential durability of the cognate late blight resistance. C_LIO_LITo identify the P. infestans effector recognized by Rpi-amr1, we screened available effector libraries and used long read and cDNA pathogen-enrichment sequencing (PenSeq) on four P. infestans isolates to explore the untested effectors. C_LIO_LIBy using SMRT and cDNA PenSeq, we identified 47 highly expressed effectors from P. infestans, including PITG_07569 which triggers a highly specific cell death response when transiently co-expressed with Rpi-amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1. C_LIO_LIHere we demonstrate that long read and cDNA PenSeq enables the identification of full-length RxLR effector families, and their expression profile. This study has revealed key insights into the evolution and polymorphism of a complex RxLR effector family that is associated with the recognition by Rpi-amr1. C_LI

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Lin, X., Song, T., Fairhead, S., Witek, K., Jouet, A., Jupe, F., Witek, A. I., Vleeshouwers, V. G. A. A., Hein, I., Jones, J. D. G.. 2020-05-14. Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen-enrichment sequencing (PenSeq). https://doi.org/10.1101/2020.05.14.095158

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