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bioRxiv · 10.1101/2020.05.12.092361

Construction of mutant heparinase I with significantly increased specific activity.

Kalinina, A.·Borschevskaya, L.·Gordeeva, T.·Patrusheva, E.·Sineokiy, S.
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Abstract

The cleavage of heparin by heparin lyases showed great potential as a cost-effective and innoxious method for producing heparin with low molecular weight (LMWH). One of the most studied and sought heparin lyase is heparinase I (HepI). However, the industrial use of HepI was largely hampered by its low specific activity and thermal stability. In this article we describe increasing in specific heparinase I activity by stepwise site-directed mutagenesis. Thus after two cycles of mutagenesis, we obtained mutant heparinase I Flavobacterium heparinum with significantly increased specific activity (25%).

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Kalinina, A., Borschevskaya, L., Gordeeva, T., Patrusheva, E., Sineokiy, S.. 2020-05-14. Construction of mutant heparinase I with significantly increased specific activity.. https://doi.org/10.1101/2020.05.12.092361

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