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bioRxiv · 10.1101/2020.05.12.081588

Testosterone enhances GLP-1 efficacy at the plasma membrane and endosomes to augment insulin secretion in male pancreatic β cells

Abstract

Male mice with elimination of the androgen receptor (AR) in islet {beta} cells ({beta}ARKO) exhibit blunted glucose-stimulated insulin secretion (GSIS), leading to hypoinsulinemia and hyperglycemia when challenged with a Western diet. Testosterone activation of an extranuclear AR in {beta} cells potentiates GSIS by amplifying the insulinotropic action of glucagon-like peptide-1 (GLP-1). Here, using a combination of {beta}ARKO and {beta} cell-selective GLP-1 receptor knockout mice and their islets, we show that AR activation in {beta} cells amplifies the insulinotropic effect of islet-derived GLP-1. In {beta} cell models expressing cAMP sensors, testosterone enhances the ability of GLP-1, but not that of glucose-dependent insulinotropic polypeptide or glucagon, to produce cAMP. Accordingly, testosterone selectively enhances the ability of GLP-1 to potentiate GSIS. Notably, testosterone enhances GLP-1 production of cAMP at the plasma membrane and endosomes. In male mouse and human islets, the insulinotropic effect of testosterone is abolished following inhibition of the membrane and endosomal cAMP-dependent protein kinase A and exchange protein activated by cAMP islet 2 pathways. Thus, membrane localization of AR enhances the ability of the GLP-1 receptor to produce cAMP, thus increasing glucose-stimulated insulin exocytosis. Significance StatementThis study reveals that testosterone, acting on the androgen receptor (AR) in insulin-producing {beta} cells amplifies the insulinotropic action of glucagon-like peptide-1 (GLP-1) by increasing GLP-1-mediated production of cAMP at the plasma membrane and endosomal compartments, to promote insulin vesicles exocytosis in human {beta} cells. This study establishes a novel biological paradigm in which membrane location of a steroid nuclear receptor enhances the ability of a G protein-coupled receptor to produce cAMP. It has exceptional clinical significance for targeted delivery of testosterone to {beta} cells in the large population of aging and androgen-deficient men who are at increased risk of diabetes.

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BibTeXRIS

Xu, W., Ashford, F., Bitsi, S., Schiffer, L., Qadir, F., Arlt, W., Tomas, A., Hodson, D., Mauvais-Jarvis, F.. 2020-05-14. Testosterone enhances GLP-1 efficacy at the plasma membrane and endosomes to augment insulin secretion in male pancreatic β cells. https://doi.org/10.1101/2020.05.12.081588

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