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bioRxiv · 10.1101/2020.05.08.083709

Computational analysis of ligand dose range thermal proteome profiles

Kurzawa, N.·Becher, I.·Sridharan, S.·Franken, H.·Mateus, A.·Anders, S.·Bantscheff, M.·Huber, W.·Savitski, M. M.
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Abstract

Detecting ligand-protein interactions in living cells is a fundamental challenge in molecular biology and drug research. Proteome-wide profiling of thermal stability as a function of ligand concentration promises to tackle this challenge. We present a statistical analysis method with reliable control of the false discovery rate and apply it to several datasets probing epigenetic drugs. We detect off-target drug engagement in unrelated protein families.

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Kurzawa, N., Becher, I., Sridharan, S., Franken, H., Mateus, A., Anders, S., Bantscheff, M., Huber, W., Savitski, M. M.. 2020-05-09. Computational analysis of ligand dose range thermal proteome profiles. https://doi.org/10.1101/2020.05.08.083709

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