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bioRxiv · 10.1101/2020.05.07.082263

VolcaNoseR - a web app for creating, exploring and sharing volcano plots

Goedhart, J.·Luijsterburg, M.
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Abstract

Comparative genome- and proteome-wide screens yield large amounts of data. To efficiently present such datasets and to simplify the identification of hits, the results are often presented in a type of scatterplot known as a volcano plot, which shows a measure of effect size versus a measure of significance. The data points with the largest effect size and a statistical significance beyond a user-defined threshold are considered as hits. Such hits are usually annotated in the plot by a label with their name. Volcano plots can represent ten thousands of data points, of which typically only a handful is annotated. The information of data that is not annotated is hardly or not accessible. To simplify access to the data and enable its re-use, we have developed an open source and online web tool with R/shiny. The web app is named VolcaNoseR and it can be used to create, explore, label and share volcano plots (https://huygens.science.uva.nl/VolcaNoseR). When the data is stored in an online data repository, the web app can retrieve that data together with user-defined settings to generate a customized, interactive volcano plot. Users can interact with the data, adjust the plot and share their modified plot together with the underlying data. Therefore, VolcaNoseR increases the transparency and re-use of large comparative genome- and proteome-wide datasets. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=143 SRC="FIGDIR/small/082263v2_ufig1.gif" ALT="Figure 1"> View larger version (26K): org.highwire.dtl.DTLVardef@3e9b17org.highwire.dtl.DTLVardef@381077org.highwire.dtl.DTLVardef@c0f6caorg.highwire.dtl.DTLVardef@8a87c2_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Goedhart, J., Luijsterburg, M.. 2020-05-07. VolcaNoseR - a web app for creating, exploring and sharing volcano plots. https://doi.org/10.1101/2020.05.07.082263

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