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bioRxiv · 10.1101/2020.05.06.081745

A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues

Glaser, A. K.·Bishop, K. W.·Barner, L. A.·Serafin, R. B.·Liu, J. T. C.
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Abstract

Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micrometer), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols and sample holders of various refractive indices. We present a hybrid system that combines a novel non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet architecture for versatile multi-scale volumetric imaging.

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Glaser, A. K., Bishop, K. W., Barner, L. A., Serafin, R. B., Liu, J. T. C.. 2020-05-08. A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues. https://doi.org/10.1101/2020.05.06.081745

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