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bioRxiv · 10.1101/2020.04.25.060855

Genome-wide chromatin binding of transcriptional corepressor Topless-related 1 in Arabidopsis

Griebel, T.·Lapin, D.·Kracher, B.·Concia, L.·Benhamed, M.·Parker, J. E.
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Abstract

Timely and specific regulation of gene expression is critical for plant responses to environmental and developmental cues. Transcriptional coregulators have emerged as important factors in gene expression control, although they lack DNA-binding domains and the mechanisms by which they are recruited to and function at the chromatin are poorly understood. Plant Topless-related 1 (TPR1), belonging to a family of transcriptional corepressors found across eukaryotes, contributes to immunity signaling in Arabidopsis thaliana and wild tobacco. We performed chromatin immunoprecipitation and sequencing (ChIP-seq) on an Arabidopsis TPR1-GFP expressing transgenic line to characterize genome-wide TPR1-chromatin associations. The analysis revealed [~]1400 genes bound by TPR1, with the majority of binding sites located at gene upstream regions. Among the TPR1 bound genes, we find not only regulators of immunity but also genes controlling growth and development. To support further analysis of TPR1-chromatin complexes and other transcriptional corepressors in plants, we provide two ways to access the processed ChIP-seq data and enable their broader use by the research community.

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Griebel, T., Lapin, D., Kracher, B., Concia, L., Benhamed, M., Parker, J. E.. 2020-04-25. Genome-wide chromatin binding of transcriptional corepressor Topless-related 1 in Arabidopsis. https://doi.org/10.1101/2020.04.25.060855

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