Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients

McKay, P. F.; Hu, K.; Blakney, A. K.; Samnuan, K.; Bouton, C. R.; Rogers, P.; Polra, K.; Lin, P. J. C.; Barbosa, C.; Tam, Y.; Shattock, R. J.

doi:10.1101/2020.04.22.055608

bioRxiv · 10.1101/2020.04.22.055608

Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients

McKay, P. F.·Hu, K.·Blakney, A. K.·Samnuan, K.·Bouton, C. R.·Rogers, P.·Polra, K.·Lin, P. J. C.·Barbosa, C.·Tam, Y.·Shattock, R. J.

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Abstract

The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine and demonstrate induction of robust neutralization of a pseudo-virus, proportional to quantity of specific IgG and of higher quantities than recovered COVID-19 patients. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic.

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McKay, P. F., Hu, K., Blakney, A. K., Samnuan, K., Bouton, C. R., Rogers, P., Polra, K., Lin, P. J. C., Barbosa, C., Tam, Y., Shattock, R. J.. 2020-04-25. Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine induces equivalent preclinical antibody titers and viral neutralization to recovered COVID-19 patients. https://doi.org/10.1101/2020.04.22.055608

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