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bioRxiv · 10.1101/2020.04.22.054502

DNA micro-disk for the management of DNA-based data storage with index and write-once-read-many (WORM) memory features

Choi, Y.·Bae, H. J.·Lee, A. C.·Choi, H.·Lee, D.·Ryu, T.·Hyun, J.·Kim, S.·Kim, H.·Song, S.-H.·Kim, K.·Park, W.·Kwon, S.
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Abstract

DNA-based data storage has attracted attention because of its higher physical density of the data and longer retention time than those of conventional digital data storage1-7. However, previous DNA-based data storage lacked index features and the data quality of storage after a single access is not preserved, obstructing its industrial use. Here, we propose DNA micro-disks, quick response (QR)-coded micro-sized disks that harbour data-encoded DNA molecules for the efficient management of DNA-based data storage. We demonstrate the two major features that previous DNA-based data storage studies could not achieve. One feature is accessing data items efficiently by indexing the data-encoded DNA library. Another is achieving write-once-read-many (WORM) memory through the immobilization of DNA molecules on the disk and their enrichment through in situ DNA production. Through these features, the reliability of DNA-based data storage was increased by allowing multiple accession of data-encoded DNA without data loss.

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Choi, Y., Bae, H. J., Lee, A. C., Choi, H., Lee, D., Ryu, T., Hyun, J., Kim, S., Kim, H., Song, S.-H., Kim, K., Park, W., Kwon, S.. 2020-04-23. DNA micro-disk for the management of DNA-based data storage with index and write-once-read-many (WORM) memory features. https://doi.org/10.1101/2020.04.22.054502

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