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bioRxiv · 10.1101/2020.04.18.048637

Swarming bacterial fronts: Dynamics and morphology of active swarm interfaces propagating through passive frictional domains

Gopinath, A.·Tamayo, J.·Zhang, Y.·Ardekani, A. M.·Patteson, A. E.
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Abstract

Swarming, a multicellular mode of flagella-based motility observed in many bacteria species, enables coordinated and rapid surface translocation, expansion and colonization. In the swarming state, bacterial films display several characteristics of active matter including intense and persistent long-ranged flocks and strong fluctuating velocity fields with significant vorticity. Swarm fronts are typically dynamically evolving interfaces. Many of these fronts separate motile active domains from passive frictional regions comprised of dead or non-motile bacteria. Here, we study the dynamics and structural features of a model active-passive interface in swarming Serratia marcescens. We expose localized regions of the swarm to high intensity wide-spectrum light thereby creating large domains of tightly packed immotile bacteria. When the light source is turned off, swarming bacteria outside this passivated region advance into this highly frictional domain and continuously reshape the interphase boundary. Combining results from Particle Image Velocimetry (PIV) and intensity based image analysis, we find that the evolving interface has quantifiable and defined roughness. Correlations between spatially separated surface fluctuations and damping of the same are influenced by the interaction of the interphase region with adjacently located and emergent collective flows. Dynamical growth exponents characterizing the spatiotemporal features of the surface are extracted and are found to differ from classically expected values for passive growth or erosion. To isolate the effects of hydrodynamic interactions generated by collective flows and that arising from steric interactions, we propose and analyze agent-based simulations with full hydrodynamics of rod-shaped, self-propelled particles. Our computations capture qualitative features of the swarm and predict correlation lengths consistent with experiments. We conclude that hydrodynamic and steric interactions enable different modes of surface dynamics, morphology and thus front invasion.

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Gopinath, A., Tamayo, J., Zhang, Y., Ardekani, A. M., Patteson, A. E.. 2020-04-18. Swarming bacterial fronts: Dynamics and morphology of active swarm interfaces propagating through passive frictional domains. https://doi.org/10.1101/2020.04.18.048637

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