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bioRxiv · 10.1101/2020.04.18.047878

Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients

Ihling, C.·Taenzler, D.·Hagemann, S.·Kehlen, A.·Huettelmaier, S.·Sinz, A.
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Abstract

Mass spectrometry (MS) can deliver valuable diagnostic data that complements genomic information and allows us to increase our current knowledge of the COVID-19 disease caused by the SARS-CoV-2 virus. We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our methodology identifies unique peptides originating from SARS-CoV-2 nucleoprotein. Building on these promising initial results, faster MS protocols can now be developed as routine diagnostic tools for COVID-19 patients. Graphical Abstract O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY Image credit (left): Gerd Altmann, Pixabay License, https://pixabay.com/illustrations/corona-coronavirus-virus-covid-19-4959447 C_FIG_DISPLAY

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Ihling, C., Taenzler, D., Hagemann, S., Kehlen, A., Huettelmaier, S., Sinz, A.. 2020-04-19. Mass Spectrometric Identification of SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients. https://doi.org/10.1101/2020.04.18.047878

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