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bioRxiv · 10.1101/2020.04.13.040170

Effects of salvianolic acid A on β-amyloid mediated toxicity in Caenorhabditis elegans model of Alzheimer's disease

Yuen, C.-W.·Abdul Halim, M.·Najimudin, N.·Azzam, G.
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Abstract

Alzheimers disease (AD) is a brain disease attributed to the accumulation of extracellular senile plaques comprising {beta}-amyloid peptide (A{beta}). In this study, a transgenic Caenorhabditis elegans containing the human beta amyloid A{beta}42 gene which exhibited paralysis when expressed, was used to study the anti-paralysis effect of salvianolic acid A. Various concentrations ranging from 1 g/ml to 100 g/ml of salvianolic acid A were tested and exhibited the highest effect on the worm at the concentration of 100 g/ml. For anti-aggregation effect, 14 g/ml salvianolic acid A (within 4 mg/ml of Danshen) showed a significant level of inhibition of the formation of A{beta} fibrils. An amount of 100 g/ml of salvianolic acid A had the potential in reducing the ROS but did not totally obliterate the ROS production in the worms. Salvianolic acid A was found to delay the paralysis of the transgenic C. elegans, decrease A{beta}42 aggregation and decreased A{beta}-induced oxidative stress.

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Yuen, C.-W., Abdul Halim, M., Najimudin, N., Azzam, G.. 2020-04-14. Effects of salvianolic acid A on β-amyloid mediated toxicity in Caenorhabditis elegans model of Alzheimer's disease. https://doi.org/10.1101/2020.04.13.040170

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