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bioRxiv · 10.1101/2020.04.09.035188

Improved synthetic lipidation-based protein translocation system for SNAP-tag fusion proteins

Yoshii, T.·Tahara, K.·Suzuki, S.·Hatano, Y.·Kuwata, K.·Tsukiji, S.
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Abstract

The ability to artificially attach lipids to specific intracellular protein targets would be a valuable approach for controlling protein localization and function in cells. We recently devised a chemogenetic method in which a SNAP-tag fusion protein can be translocated from the cytoplasm to the plasma membrane by post-translationally and covalently conjugating a synthetic lipopeptide in cells. However, the first-generation system lacked general applicability. Herein, we present an improved synthetic lipidation system that enables efficient plasma membrane translocation of SNAP-tag fusion proteins in cells. This second-generation system is now applicable to the control of various cell-signaling molecules, offering a new and useful research tool in chemical biology and synthetic biology.

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Yoshii, T., Tahara, K., Suzuki, S., Hatano, Y., Kuwata, K., Tsukiji, S.. 2020-04-10. Improved synthetic lipidation-based protein translocation system for SNAP-tag fusion proteins. https://doi.org/10.1101/2020.04.09.035188

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