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bioRxiv · 10.1101/2020.04.07.029009

Disseminating cells in human tumours acquire an EMT stem cell state that is predictive of metastasis

Youssef, G.·Gammon, L.·Ambler, L.·Wicker, B.·Patel, S.·Cottom, H.·Piper, K.·Mackenzie, I. C.·Philpott, M. P.·Biddle, A.
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Abstract

Cancer stem cells (CSCs) undergo epithelial-mesenchymal transition (EMT) to drive metastatic dissemination in experimental cancer models. However, tumour cells undergoing EMT have not been observed disseminating into the tissue surrounding human tumour specimens, leaving the relevance to human cancer uncertain. We have previously identified both EpCAM and CD24 as markers of EMT CSCs with enhanced plasticity. This afforded the opportunity to investigate whether retention of EpCAM and CD24 alongside upregulation of the EMT marker Vimentin can identify disseminating EMT CSCs in human tumours. Examining disseminating tumour cells in over 12,000 imaging fields from 84 human oral cancer specimens, we see a significant enrichment of single EpCAM, CD24 and Vimentin co-stained cells disseminating beyond the tumour body in metastatic specimens. Through training an artificial neural network, these predict metastasis with high accuracy (cross-validated accuracy of 87-89%). In this study, we have observed single disseminating EMT CSCs in human oral cancer specimens, and these are highly predictive of metastatic disease.

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Youssef, G., Gammon, L., Ambler, L., Wicker, B., Patel, S., Cottom, H., Piper, K., Mackenzie, I. C., Philpott, M. P., Biddle, A.. 2020-04-08. Disseminating cells in human tumours acquire an EMT stem cell state that is predictive of metastasis. https://doi.org/10.1101/2020.04.07.029009

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