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bioRxiv · 10.1101/2020.04.06.028597

Genetically Defined, Syngeneic Organoid Platform for Developing Combination Therapies for Ovarian Cancer

Neel, B. G.·zhang, s.·Iyer, S.·Ran, H.·wei, W.·Foster, C.·Weinberg, R. A.
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Abstract

The paucity of genetically informed, immune-competent tumor models impedes evaluation of conventional, targeted, and immune therapies. By engineering mouse fallopian tube (FT) organoids using lentiviral gene transduction and/or CRISPR/Cas9 mutagenesis, we generated multiple high grade serous ovarian carcinoma (HGSOC) models exhibiting mutational combinations seen in patients. Detailed analysis of homologous recombination (HR)-proficient (Tp53-/-;Ccne1OE;Akt2OE; KrasOE), HR-deficient (Tp53-/-;Brca1-/-;MycOE) and unclassified (Tp53-/-;Pten-/-;Nf1-/-) organoids revealed differences in in vitro properties and tumorigenicity. Tumorigenic organoids had variable sensitivity to HGSOC chemotherapeutics and evoked distinct immune microenvironments. These findings enabled development of a chemotherapy/immunotherapy regimen that yielded durable, T-cell dependent responses in Tp53-/-;Ccne1OE;Akt2OE;Kras HGSOC; by contrast, Tp53-/-;Pten-/-;Nf1-/- tumors failed to respond. Genotype-informed, syngeneic organoid models could provide an improved platform for rapid evaluation of tumor biology and therapeutics. HIGHLIGHTSO_LIOrthotopic injection of genetically defined fallopian tube organoids yield HGSOC. C_LIO_LIOvarian tumors with different genotypes evoke distinct immune microenvironments C_LIO_LICombining Gemcitabine, anti-PD-L1, and anti-CTLA-4 result in complete responses in Tp53-/-;Ccne1OE;Akt2OE;KrasOE organoid-derived HGSOC C_LIO_LITherapeutic response is tumor genotype-specific C_LI

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Neel, B. G., zhang, s., Iyer, S., Ran, H., wei, W., Foster, C., Weinberg, R. A.. 2020-04-07. Genetically Defined, Syngeneic Organoid Platform for Developing Combination Therapies for Ovarian Cancer. https://doi.org/10.1101/2020.04.06.028597

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