Cellular senescence and quiescence are associated with altered ribosomal RNA methylation and processing

Yang, G.; Heisenberger, C.; Kos-Braun, I. C.; Polacek, N.; Grillari, J.; Schosserer, M.; Kos, M.

doi:10.1101/2020.04.01.019653

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bioRxiv · 10.1101/2020.04.01.019653

Cellular senescence and quiescence are associated with altered ribosomal RNA methylation and processing

Yang, G.·Heisenberger, C.·Kos-Braun, I. C.·Polacek, N.·Grillari, J.·Schosserer, M.·Kos, M.

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Abstract

The 2-O-methylation (2-O-Me) of ribosomal RNA (rRNA) shows plasticity potentially associated with specific cell phenotypes. We used RiboMeth-seq profiling to reveal 2-O-Me patterns specific to stress induced premature senescent (SIPS), quiescent and proliferating human dermal fibroblasts. The altered methylation levels in SIPS and quiescence partially correlated with the expression of specific snoRNAs but not fibrillarin. Senescence and quiescence were accompanied by a trend towards preferred usage of one of the two alternative ribosome biogenesis pathways.

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Yang, G., Heisenberger, C., Kos-Braun, I. C., Polacek, N., Grillari, J., Schosserer, M., Kos, M.. 2020-04-02. Cellular senescence and quiescence are associated with altered ribosomal RNA methylation and processing. https://doi.org/10.1101/2020.04.01.019653

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