Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein

Poh, C. M.; Carissimo, G.; Bei, W.; Amrun, S. N.; Lee, C. Y.-P.; Chee, R. S.-L.; Yeo, N. K.-W.; Lee, W.-H.; Leo, Y.-S.; Chen, M. I.-C.; Tan, S.-Y.; Chai, L. Y. A.; Kalimuddin, S.; Thien, S.-Y.; Young, B. E.; Lye, D. C.; Wang, C.-I.; Renia, L.; Ng, L. F.

doi:10.1101/2020.03.30.015461

bioRxiv · 10.1101/2020.03.30.015461

Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein

Poh, C. M.·Carissimo, G.·Bei, W.·Amrun, S. N.·Lee, C. Y.-P.·Chee, R. S.-L.·Yeo, N. K.-W.·Lee, W.-H.·Leo, Y.-S.·Chen, M. I.-C.·Tan, S.-Y.·Chai, L. Y. A.·Kalimuddin, S.·Thien, S.-Y.·Young, B. E.·Lye, D. C.·Wang, C.-I.·Renia, L.·Ng, L. F.

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Abstract

The ongoing SARS-CoV-2 pandemic demands rapid identification of immunogenic targets for the design of efficient vaccines and serological detection tools. In this report, using pools of overlapping linear peptides and functional assays, we present two immunodominant regions on the spike glycoprotein that were highly recognized by neutralizing antibodies in the sera of COVID-19 convalescent patients. One is highly specific to SARS-CoV-2, and the other is a potential pan-coronavirus target.

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Poh, C. M., Carissimo, G., Bei, W., Amrun, S. N., Lee, C. Y.-P., Chee, R. S.-L., Yeo, N. K.-W., Lee, W.-H., Leo, Y.-S., Chen, M. I.-C., Tan, S.-Y., Chai, L. Y. A., Kalimuddin, S., Thien, S.-Y., Young, B. E., Lye, D. C., Wang, C.-I., Renia, L., Ng, L. F.. 2020-03-31. Potent neutralizing antibodies in the sera of convalescent COVID-19 patients are directed against conserved linear epitopes on the SARS-CoV-2 spike protein. https://doi.org/10.1101/2020.03.30.015461

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