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bioRxiv · 10.1101/2020.03.26.010108

A novel full-reduced HMGB1 and kartogenin-containing bio-active scaffold for meniscus regeneration

Xu, H.·Dai, Z.·Zou, X.·Xia, P.·ABOUDI, M. A.·Wang, J.·Lam Sung Foon, W. A.·Huang, H.
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Abstract

A novel bio-active scaffold for enhancing wounded meniscus healing has been developed by combination of High mobility group box 1 protein (HMGB1) and kartogenin (KGN) with alginate gel. The properties of the bio-active scaffold were also investigated using an in vitro cell culture model and in vivo rat wounded meniscus healing model. This HMGB1-KGN-containing bioactive scaffold released HMGB1 and KGN into wound area and kept high concentrations of HMGB1 and KGN in the system for more than two weeks. This HMGB1-KGN-containing bioactive scaffold also activated rat bone marrow stem cells (BMSCs) from G0 to GAlert stage and promoted cell proliferation as evidenced by 5-bromo-2-deoxyuridine (BrdU) incorporation testing. Our results also demonstrated that the HMGB1-KGN-containing bioactive scaffold induced cell migration in vitro and recruited the cells to wound area to promote wounded rat meniscus healing in vivo.

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Xu, H., Dai, Z., Zou, X., Xia, P., ABOUDI, M. A., Wang, J., Lam Sung Foon, W. A., Huang, H.. 2020-03-29. A novel full-reduced HMGB1 and kartogenin-containing bio-active scaffold for meniscus regeneration. https://doi.org/10.1101/2020.03.26.010108

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