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bioRxiv · 10.1101/2020.03.18.995332

Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue

Alsema, A. M.·Jiang, Q.·Kracht, L.·Gerrits, E.·Dubbelaar, M. L.·Miedema, A.·Brouwer, N.·Woodbury, M.·Wachter, A.·Xi, H. S.·Moeller, T.·Biber, K. P.·Kooistra, S. M.·Boddeke, E. H. W. G. M.·Eggen, B. J. L.
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Abstract

Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimers disease. Here, we investigated microglia transcriptomes at bulk and single cell level in non-demented elderly and AD donors using acute human post-mortem cortical brain samples. We identified 9 human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.

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Alsema, A. M., Jiang, Q., Kracht, L., Gerrits, E., Dubbelaar, M. L., Miedema, A., Brouwer, N., Woodbury, M., Wachter, A., Xi, H. S., Moeller, T., Biber, K. P., Kooistra, S. M., Boddeke, E. H. W. G. M., Eggen, B. J. L.. 2020-03-19. Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue. https://doi.org/10.1101/2020.03.18.995332

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