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bioRxiv · 10.1101/2020.03.10.986273

γ-H2AX is present at mouse meiotic kinetochores

Guajardo, A.·Viera, A.·Parra, M. T.·Martinez Valdivia, M. J.·Rufas, J. S.·Suja, J. A.
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Abstract

The histone variant H2AX phosphorylated on serine 139, named {gamma}-H2AX, is a canonical DNA double-strand breaks marker. During mammalian meiotic prophase I, {gamma}-H2AX participates in meiotic recombination, meiotic sex chromosome inactivation and meiotic silencing of unsynapsed chromatin. In this study, we have analyzed the distribution of {gamma}-H2AX during male mouse meiosis by immunofluorescence on spread and squashed spermatocytes. We have found that {gamma}-H2AX locates at the inner kinetochore plate of meiotic kinetochores in both meiotic divisions. Therefore our results, for the first time, uncover a novel role for {gamma}-H2AX at mammalian meiotic kinetochores.

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Guajardo, A., Viera, A., Parra, M. T., Martinez Valdivia, M. J., Rufas, J. S., Suja, J. A.. 2020-03-11. γ-H2AX is present at mouse meiotic kinetochores. https://doi.org/10.1101/2020.03.10.986273

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