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bioRxiv · 10.1101/2020.03.05.974956

Processive and stochastic CHH methylation by plant DRM2 and CMT2 revealed by single-read methylome analysis

Harris, K. D.·Zemach, A.
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Abstract

Cytosine methylome data is commonly generated through next-generation sequencing (NGS). Analyses of this data average methylation states of individual reads. We propose an alternate method of analysing single-read methylome data. Using this method, we identified patterns that relate to the mechanism of two plant non-CG methylating enzymes, DRM2 and CMT2: DRM2 has higher processivity than CMT2, and DRM2-methylated regions have higher variation among cells. Based on these patterns, we developed a classifier that predicts enzyme activity in different species and tissues. To facilitate further single-read analyses, we developed a genome browser optimised for visualising and analysing NGS data at single-read resolution.

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Harris, K. D., Zemach, A.. 2020-03-06. Processive and stochastic CHH methylation by plant DRM2 and CMT2 revealed by single-read methylome analysis. https://doi.org/10.1101/2020.03.05.974956

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