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bioRxiv · 10.1101/2020.03.02.968388

Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2

Kim, Y.·Jedrzejczak, R.·Maltseva, N. I.·Endres, M.·Godzik, A.·Michalska, K.·Joachimiak, A.
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Abstract

Severe Acute Respiratory Syndrome Coronavirus 2 is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS- and MERS-CoVs the detailed information about SARS-CoV-2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies and antivirals. We applied high-throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS-CoV-2 proteins and structures. Here we report the high-resolution crystal structure of endoribonuclease Nsp15/NendoU from SARS-CoV-2 - a virus causing current world-wide epidemics. We compare this structure with previously reported models of Nsp15 from SARS and MERS coronaviruses.

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Kim, Y., Jedrzejczak, R., Maltseva, N. I., Endres, M., Godzik, A., Michalska, K., Joachimiak, A.. 2020-03-03. Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. https://doi.org/10.1101/2020.03.02.968388

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