CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering

Anderson, D. P.; Benns, H. J.; Tate, E. W.; Child, M. A.

doi:10.1101/2020.02.26.965889

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bioRxiv · 10.1101/2020.02.26.965889

CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering

Anderson, D. P.·Benns, H. J.·Tate, E. W.·Child, M. A.

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Abstract

Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time consuming post-hoc curation, simplifying gRNA outputs, and reducing CPU times.

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Anderson, D. P., Benns, H. J., Tate, E. W., Child, M. A.. 2020-02-26. CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering. https://doi.org/10.1101/2020.02.26.965889

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