Single cell sequencing of the small and AT-skewed genome of malaria parasites

Liu, S.; Huckaby, A. C.; Brown, A. C.; Moore, C. C.; Burbulis, I. E.; McConnell, M. J.; Guler, J. L.

doi:10.1101/2020.02.21.960039

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bioRxiv · 10.1101/2020.02.21.960039

Single cell sequencing of the small and AT-skewed genome of malaria parasites

Liu, S.·Huckaby, A. C.·Brown, A. C.·Moore, C. C.·Burbulis, I. E.·McConnell, M. J.·Guler, J. L.

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Abstract

Single cell genomics is a rapidly advancing field; however, most techniques are designed for mammalian cells. Here, we present a single cell sequencing pipeline for the intracellular parasite, Plasmodium falciparum, which harbors a relatively small genome with an extremely skewed base content. Through optimization of a quasi-linear genome amplification method, we achieve better targeting of the parasite genome over contaminants and generate coverage levels that allow detection of relatively small copy number variations on a single cell level. These improvements are important for expanding accessibility of single cell approaches to new organisms and for improving the study of adaptive mechanisms.

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Liu, S., Huckaby, A. C., Brown, A. C., Moore, C. C., Burbulis, I. E., McConnell, M. J., Guler, J. L.. 2020-02-24. Single cell sequencing of the small and AT-skewed genome of malaria parasites. https://doi.org/10.1101/2020.02.21.960039

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