Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification

Wilken, M. S.; Ciarlo, C.; Pearl, J.; Bloom, J.; Schanzer, E.; Liao, H.; Boyken, S. E.; Van Biber, B.; Queitsch, K.; Heberlein, G.; Federation, A.; Acosta, R.; Vong, S.; Otterman, E.; Dunn, D.; Wang, H.; Zrazhevskey, P.; Nandakumar, V.; Bates, D.; Sandstrom, R.; Chen, Z.; Urnov, F. D.; Baker, D.; Funnell, A.; Green, S.; Stamatoyannopoulos, J. A.

doi:10.1101/2020.02.19.956730

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bioRxiv · 10.1101/2020.02.19.956730

Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification

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Abstract

Human genes are regulated quantitatively, yet the ability to specify the expression level of a native gene accurately and specifically using a defined reagent has remained elusive. Here we show that precise targeting of KRAB repressive domain within regulatory DNA unlocks an endogenous quantitative dial that can be engaged at nucleotide resolution to program expression levels across a wide physiologic range, with single-gene specificity and high reproducibly in primary cells.

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Wilken, M. S., Ciarlo, C., Pearl, J., Bloom, J., Schanzer, E., Liao, H., Boyken, S. E., Van Biber, B., Queitsch, K., Heberlein, G., Federation, A., Acosta, R., Vong, S., Otterman, E., Dunn, D., Wang, H., Zrazhevskey, P., Nandakumar, V., Bates, D., Sandstrom, R., Chen, Z., Urnov, F. D., Baker, D., Funnell, A., Green, S., Stamatoyannopoulos, J. A.. 2020-02-20. Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification. https://doi.org/10.1101/2020.02.19.956730

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