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bioRxiv · 10.1101/2020.02.18.955195

Structure and immune recognition of the porcine epidemic diarrhea virus spike protein

Kirchdoerfer, R.·Bhandari, M.·Martini, O.·Sewell, L. M.·Bangaru, S.·Yoon, K.-J.·Ward, A.
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Abstract

Porcine epidemic diarrhea virus is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a key target for the host antibody response and a good candidate for a protein-based vaccine immunogen. We used electron microscopy to evaluate the PEDV spike structure, as well as pig polyclonal antibody responses to viral infection. The structure of the PEDV spike reveals a configuration similar to that of HuCoV-NL63. Several PEDV protein-protein interfaces are mediated by non-protein components including a glycan at Asn264 and two bound palmitoleic acid molecules. The polyclonal antibody response to PEDV infection shows a dominance of epitopes in the S1 region. This structural and immune characterization provides new insights into coronavirus spike stability determinants and explores the immune landscape of viral spike proteins.

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Kirchdoerfer, R., Bhandari, M., Martini, O., Sewell, L. M., Bangaru, S., Yoon, K.-J., Ward, A.. 2020-02-19. Structure and immune recognition of the porcine epidemic diarrhea virus spike protein. https://doi.org/10.1101/2020.02.18.955195

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