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bioRxiv · 10.1101/2020.02.18.954610

CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization

Feng, Y.·Wang, Y.·Yang, C.·Naseri, A.·Pederson, T.·Zheng, J.·Xiao, X.·Zhang, S.·Xie, W.·Ma, H.
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Abstract

Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1 condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1 condensates possibly driven by phase separation.

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Feng, Y., Wang, Y., Yang, C., Naseri, A., Pederson, T., Zheng, J., Xiao, X., Zhang, S., Xie, W., Ma, H.. 2020-02-19. CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization. https://doi.org/10.1101/2020.02.18.954610

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