Oocytes, a single cell and a tissue

Wu, D.; Dean, J.

doi:10.1101/2020.02.17.952929

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bioRxiv · 10.1101/2020.02.17.952929

Oocytes, a single cell and a tissue

Wu, D.·Dean, J.

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Abstract

Development of single cell sequencing allows detailing the transcriptome of individual oocytes. Here, we compare different RNA-seq datasets from single and pooled mouse oocytes and show higher reproducibility using single oocyte RNA-seq. We further demonstrate that UMI (unique molecular identifiers) based and other deduplication methods are limited in their ability to improve the precision of these datasets. Finally, for normalization of sample differences in cross-stage comparisons, we propose that external spike-in molecules are comparable to using the endogenous genes stably expressed during oocyte maturation. The ability to normalize data among single cells provides insight into the heterogeneity of mouse oocytes.

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Wu, D., Dean, J.. 2020-02-17. Oocytes, a single cell and a tissue. https://doi.org/10.1101/2020.02.17.952929

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