LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

Joshi, A.; Das, S. K.; Bisht, L.; Sakat, S.; Sharma, V.; Sharma, N.; Joshi, K.; Verma, S.; Sharma, V. K.; Balkrishna, A.; Joshi, C. S.

doi:10.1101/2020.02.15.950865

bioRxiv · 10.1101/2020.02.15.950865

LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

Joshi, A.·Das, S. K.·Bisht, L.·Sakat, S.·Sharma, V.·Sharma, N.·Joshi, K.·Verma, S.·Sharma, V. K.·Balkrishna, A.·Joshi, C. S.

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Abstract

The incidence rate of diabetes mellitus is increasing worldwide. Herbal formulations have recently gained importance as an alternative therapeutic option in controlling diabetes without causing any side effects. In the present study, we have demonstrated maintenance of glycemic homeostasis and anti-adipogenic potential of a herbo-mineral formulation Divya MadhuKalp (DMK). Initially, we evaluated the presence of bioactive compounds in DMK using LC/MS-QToF analysis. In-vitro analysis of DMK in L6 (skeletal muscle) cells showed a significant increase in cellular glucose uptake. Similarly, a human equivalent dose of DMK significantly reduced blood glucose level in normoglycemic and oral glucose tolerance rat model. DMK extract also inhibited formation of advanced glycation end product and showed anti--glucosidase activity. Further analysis of DMK in 3T3 L1 pre-adipocytes demonstrated anti-adipogenic activity through reduction in intracellular lipid accumulation and triglyceride contents along with downregulation of major adipogenic transcriptional factors (PPAR-{gamma} and C/EBP) and, adipocytes marker genes (LPL, AP2 and adiponectin). In conclusion, DMK exhibited anti-diabetic and anti-adipogenic activities by synergistic effect of its bioactive compounds and can be considered as a potent herbo-mineral formulation for treating metabolic diseases.

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Joshi, A., Das, S. K., Bisht, L., Sakat, S., Sharma, V., Sharma, N., Joshi, K., Verma, S., Sharma, V. K., Balkrishna, A., Joshi, C. S.. 2020-02-15. LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation. https://doi.org/10.1101/2020.02.15.950865

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