2MV/cm Pulsed Electric Fields Promote Transthyretin Amyloid Disintegration

Urabe, G.; Sato, T.; Nakamura, G.; Kobashigawa, Y.; Morioka, H.; Katsuki, S.

doi:10.1101/2020.02.15.950501

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bioRxiv · 10.1101/2020.02.15.950501

2MV/cm Pulsed Electric Fields Promote Transthyretin Amyloid Disintegration

Urabe, G.·Sato, T.·Nakamura, G.·Kobashigawa, Y.·Morioka, H.·Katsuki, S.

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Abstract

Exposing transthyretin amyloid to 1000 sub-nanosecond 2 MV/cm pulsed electric fields (PEFs) promotes both amyloid disassembly and amyloid-derived transthyretin disintegration. The process produced no change in pH, and the resulting temperature increase was less than 1 {degrees}C. We conclude that PEFs physical effects facilitate amyloid disassembly, rather than thermal or chemical effects, and provoke amyloid-derived transthyretin disintegration, the latter of which is reported here for the first time.

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Urabe, G., Sato, T., Nakamura, G., Kobashigawa, Y., Morioka, H., Katsuki, S.. 2020-02-15. 2MV/cm Pulsed Electric Fields Promote Transthyretin Amyloid Disintegration. https://doi.org/10.1101/2020.02.15.950501

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