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bioRxiv · 10.1101/2020.02.14.949024

Steroid hormone agonists reduce female fitness in insecticide-resistant Anopheles populations

Brown, F.·Paton, D. G.·Catteruccia, F.·Ranson, H.·Ingham, V. A.
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Abstract

Insecticide based vector control tools such as insecticide treated bednets and indoor residual spraying represent the cornerstones of malaria control programs. Resistance to chemistries used in these programs is now widespread and represents a significant threat to the gains seen in reducing malaria-related morbidity and mortality. Recently, disruption of the 20-hydroxyecdysone steroid hormone pathway was shown to reduce Plasmodium development time and significantly reduce both longevity and egg production in a laboratory susceptible Anopheles gambiae population. Here, we demonstrate that disruption of this pathway by application of methoxyfenozide (MET) to insecticide resistant An. coluzzii, An. gambiae sl and An. funestus populations significantly reduces egg production in both topical and tarsal application. Moreover, MET reduces adult longevity when applied topically, and tarsally after blood feeding. As the cytochrome p450s elevated in pyrethroid resistant Anopheles only bind MET very weakly, this compound is unlikely to be subject to cross-resistance in a field-based setting. Manipulation of this hormonal signalling pathway therefore represents a potential complementary approach to current malaria control strategies, particularly in areas where high levels of insecticide resistance are compromising existing tools.

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Brown, F., Paton, D. G., Catteruccia, F., Ranson, H., Ingham, V. A.. 2020-02-14. Steroid hormone agonists reduce female fitness in insecticide-resistant Anopheles populations. https://doi.org/10.1101/2020.02.14.949024

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